Study of Neonatal Exposure to Estrogenic and Androgenic Endocrine Disruptors by Normal-Phase HPLC

E-ISSN： 2210-304x|2|2|126-131

ISSN： 2210-3031

Source： Drug Delivery Letters, Vol.2, Iss.2, 2012-06, pp. : 126-131

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Abstract

Neonatal exposure to estrogenic and androgenic endocrine disruptors induces developmental abnormalities in the female reproductive system. To investigate whether neonatal exposure affects oogenesis in juvenile and pubertal ovary, Sprague-Dawley rat pups were given estrogen or various endocrine disruptors by a single injection on the day of birth at concentrations ranging between 2 mM to 40 mM, and sacrificed on day 21 (juvenile) or 50 (puberty). The ovaries were weighed and examined histologically at each stage. Further, 17&bgr;-estradiol synthesis of oogenesis was analyzed using normal-phase high-performance liquid chromatography. Neonatal exposure to 17 &bgr;-estradiol, diethylstilbestrol and estrogen receptor inhibitors significantly reduced ovary weights, although estrogen receptor inhibitors were completely restored by 17 &bgr;-estradiol synthesis during puberty.