Publisher： John Wiley & Sons Inc
E-ISSN： 1521-4125|38|8|1444-1451
ISSN： 0930-7516
Source： CHEMICAL ENGINEERING & TECHNOLOGY (CET), Vol.38, Iss.8, 2015-08, pp. : 1444-1451
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
AbstractThe preparation of chitosan‐coated magnetic nanoparticles (MNPs) and covalent immobilization of α‐amylase for starch hydrolysis was investigated. Surface morphology, chemical composition, and structural characteristics of the MNPs were analyzed by scanning electron microscopy, energy dispersion spectrometry, and X‐ray diffractometry, respectively. Surface functional groups of MNPs, chitosan‐coated MNPs, and α‐amylase‐immobilized MNPs were characterized by Fourier transform infrared spectroscopy. Response surface methodology based on three levels was implemented to optimize three immobilization conditions and a regression model was developed. α‐Amylase‐immobilized MNPs provided better stability towards pH and temperature. The prepared thermostable nanobiocatalyst is well‐suited for industrial processes involving starch hydrolysis.
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