Publisher： John Wiley & Sons Inc
E-ISSN： 1346-8138|19|11|793-796
ISSN： 0385-2407
Source： THE JOURNAL OF DERMATOLOGY, Vol.19, Iss.11, 1992-11, pp. : 793-796
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Abstract
AbstractHuman hair follicles were isolated from the scalp by dispase and collagenase treatment and dispersed into a cell suspension by trypsin. These cells proliferated well and could be subcultured 7 to 8 times. The medium used was MCDB 153 HAA medium further supplemented with some amino acids, hydrocortisone, insulin, EGF, and bovine brain extract. The concentration of Ca++ was adjusted to 0.1 mM. Immunohistochemically, these cells were proved to possess keratins specific to hair forming cells.
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