Chemical synthesis and biophysical applications of membrane proteins

E-ISSN： 1099-1387|21|7|540-549

ISSN： 1075-2617

Source： JOURNAL OF PEPTIDE SCIENCE (ELECTRONIC), Vol.21, Iss.7, 2015-07, pp. : 540-549

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Abstract

Chemical synthesis or semi‐synthesis of membrane proteins can provide unique molecular tools, such as site‐specific isotope labeling or post‐translationally modified membrane proteins to gain insight into their biophysical and functional characteristics. However, during preparation, purification, and ligation of transmembrane peptides, tremendous challenges are encountered owing to their hydrophobic nature. This review focuses on the recent advances in chemical synthesis strategies of membrane proteins. These strategies help to solubilize the hydrophobic transmembrane peptide sequences under standard purification and chemical ligation conditions to improve their handling properties. Biophysical and functional studies of synthetic membrane proteins are reviewed as well. Copyright © 2014 European Peptide Society and John Wiley & Sons, Ltd.