Publisher: John Wiley & Sons Inc
E-ISSN: 1439-7633|1439-4227|14|2073-2079
ISSN: 1439-4227
Source: CHEMBIOCHEM, Vol.1439-4227, Iss.14, 2015-09, pp. : 2073-2079
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
AbstractWater‐in‐oil (w/o) emulsions are used as a cellular model because of their unique cell‐like architecture. Previous works showed the capability of eukaryotic‐cell‐sized w/o droplets (5–50 μm) to support protein synthesis efficiently; however data about smaller w/o compartments (<1 μm) are lacking. This work focuses on the biosynthesis of the enhanced green fluorescent protein (EGFP) inside sub‐micrometric lecithin‐based w/o droplets (0.8–1 μm) and on its dependence on the compartments’ dynamic properties in terms of solute exchange mechanisms. We demonstrated that protein synthesis is strongly affected by the nature of the lipid interface. These findings could be of value and interest for both basic and applied research.
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