Publisher： John Wiley & Sons Inc
E-ISSN： 1439-0426|31|S1|136-146
ISSN： 0175-8659
Source： JOURNAL OF APPLIED ICHTHYOLOGY (ELECTRONIC), Vol.31, Iss.S1, 2015-06, pp. : 136-146
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Abstract
SummaryThe objective of the study was to investigate the effects of a static magnetic field on the trout sperm motility parameters (CASA), DNA integrity (comet assay) and morphology (SEM). The fertilisation rate was also assessed. Exposing the trout spermatozoa to a static magnetic field (1 mT, 5 mT, 10 mT) prolonged the sperm motility to 12 days (288 h), while the control sperm remained motile for only 3 days (72 h). Analysis of fragmentation of genetic material in the spermatozoon head revealed no effects from the studied magnetic fields. After a 30‐min exposure of spermatozoa to the magnetic field, the proportion of fertilised eggs was already 12.7% (1 mT) to 15% (10 mT), higher than that in the control variant. After a 24‐h storage in the magnetic field the difference was even larger – <1% of eggs were fertilised in the control; in the magnetic field the proportion of fertilised eggs increased to 11.4 at 3 mT and 22.1 at 1 mT. The results demonstrate that short‐term storage of sperm in a static magnetic field of low intensity has a positive effect on the sperm motility parameters (not causing DNA fragmentation), which in turn improves the effectiveness of fertilisation and hatching. This suggests that this method, after more comprehensive studies, might be used for short‐term storage of trout sperm.
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