Crystallization and preliminary crystallographic analysis of the Magnetospirillum magneticum AMB‐1 and M. gryphiswaldense MSR‐1 magnetosome‐associated proteins MamA

Publisher： John Wiley & Sons Inc

E-ISSN： 1744-3091|66|7|824-827

ISSN： 1744-3091

Source： Acta Crystallographica Section F, Vol.66, Iss.7, 2010-06, pp. : 824-827

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Abstract

MamA is a unique magnetosome‐associated protein that is predicted to contain six sequential tetratricopeptide‐repeat (TPR) motifs. The TPR structural motif serves as a template for protein–protein interactions and mediates the assembly of multi‐protein complexes. Here, the crystallization and preliminary X‐ray analysis of recombinant and purified Magnetospirillum magneticum and M. gryphiswaldense MamA are reported for the first time. M. gryphiswaldense MamAΔ41 crystallized in the tetragonal space group P4₁2₁2 or P4₃2₁2, with unit‐cell parameters a = b = 58.88, c = 144.09 Å. M. magneticum MamAΔ41 crystallized in the orthorhombic space group P2₁2₁2₁, with unit‐cell parameters a = 44.75, b = 76.19, c = 105.05 Å. X‐ray diffraction data were collected to resolutions of 2.0 and 1.95 Å, respectively.