Comparative study of stemness characteristics of mesenchymal cells from bone marrow of children and adults

Author： Choumerianou Despoina M. Martimianaki Georgia Stiakaki Eftichia Kalmanti Lida Kalmanti Maria Dimitriou Helen

ISSN： 1465-3249

Source： Cytotherapy, Vol.12, Iss.7, 2010-11, pp. : 881-887

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Abstract

AbstractBackground aims. Age-related changes that could affect the biologic features of mesenchymal stromal cells (MSC), such as a decrease in proliferation and osteoblast differentiation capacity and an increase of senescence markers and apoptosis, have been reported recently. The aim of this study was the evaluation of age-related characteristics and the correlation of age with the functional properties of MSC. Methods. The doubling time (DT), colony-forming unit–fibroblast (CFU-F) colonies and surface antigen expression of MSC isolated from bone marrow (BM) of children (C-MSC) were compared with those from adults (A-MSC). The expression of Oct-4 and Nanog transcripts and the relative telomere length were evaluated in both groups. Results. DT values were lower in C-MSC compared with A-MSC, and a higher CFU-F count was observed in children. However, the expression of Oct-4 and Nanog did not differ between C-MSC and A-MSC and was not correlated with the proliferative capacity. The telomere length was significantly higher in C-MSC compared with A-MSC. Conclusions. These data suggest that children's BM-derived MSC could be a more advantageous source of these cells for tissue engineering and cell therapy.