Publisher： John Wiley & Sons Inc
E-ISSN： 1447-0594|15|6|804-810
ISSN： 1444-1586
Source： GERIATRICS & GERONTOLOGY INTERNATIONAL, Vol.15, Iss.6, 2015-06, pp. : 804-810
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
AimSenescence marker protein‐30 (SMP30)/gluconolactonase (GNL) is an age‐associated protein in that its presence decreases with aging. Here, we used immunohistochemical analysis to investigate the changes of SMP30/GNL in individual cells of the liver from progressively aged mice.MethodsMale C57BL/6 strain mice at 1, 3, 6, 12, 24 and 30 months‐of‐age were the source of hepatic cells used to detect SMP30/GNL. Liver sections from these mice were subjected to immunohistochemical staining with anti‐SMP30/GNL antibody. For immunofluorescent staining, primary cultured hepatocytes from mice at various ages were stained with SMP30/GNL and albumin.ResultsIn liver cells from mice of all ages, SMP30/GNL staining appeared in some but not all parenchymal cells, and localized in both the nuclei and cytoplasm. Moreover, SMP30/GNL‐positive staining of parenchymal cells was present only around central vein areas, but not at sites of portal veins. Furthermore, the number of SMP30/GNL‐positive cells increased as mice aged from 1 to 12 months, then decreased from the 12th to 24th month. Results were similar in primary cultured hepatocytes from mice of various ages.ConclusionsSMP30/GNL‐positive cells localized mainly around the central veins in the livers of mice and decreased numerically with aging, although there was no age‐related change in counts of albumin‐positive cells. SMP30/GNL protein occupied the nuclei and cytoplasm. Therefore, nuclear SMP30/GNL protein might be a regulatory factor specific for genes whose expression governs transcription and the aging process. Geriatr Gerontol Int 2015; 15: 804–810.
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