Author： Yoshioka T Morimoto Y Iwagaki H Itoh H Saito S Kobayashi N Yagi T Tanaka N
Publisher： Field House Publishing
ISSN： 0300-0605
Source： The Journal of International Medical Research, Vol.29, Iss.5, 2001-09, pp. : 409-420
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Abstract
This study examined, in human cancer cell lines, the pattern of cytokine production stimulated by lipopoly-saccharide (LPS), a major component of the outer surface of Gram-negative bacteria, and characterized the expression pattern of CD14, cell surface LPS receptor antigen, and toll-like receptors (TLRs), which appear to be key regulators of the innate immune response system. Two colon cancer cell lines (DLD and LoVo), a hepatocellular carcinoma cell line and a myelomonocytic cell line were incubated with LPS for 0 – 72 h, and transforming growth factor (TGF) β1 and β2, hepatocyte growth factor (HGF) and interleukins 6, 8 and 15 were assayed. The only changes induced by incubation with LPS were significant increases in TGFβ1 production at 12 h, and in HGF production at 72 h, in LPS-stimulated DLD cells, and significant increases in TGFβ2 production after 12 h and in HGF after 72 h in LoVo cells. Using reverse transcriptase–polymerase chain reaction analysis, expression of CD14 and TLR-2 mRNA was detected in DLD and LoVo cells, and expression of TLR-4 mRNA was detected in PLC/PRF/5 and KG-1 cells. These results suggest that LPS induces TGFβ and HGF production mediated by CD14/TLR-2 in cultured human colon cancer cell lines.
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