Effect of Small Interfering RNA Targeting Wild-type FLT3 in Acute Myeloid Leukaemia Cells In Vitro and In Vivo

Author： Wang C-M Sheng G-Y Lu J Xie L Bai S-T Xu X-J Liu Y-F

ISSN： 0300-0605

Source： The Journal of International Medical Research, Vol.39, Iss.5, 2011-09, pp. : 1661-1674

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Abstract

This study investigated the effect of using small interfering RNA (siRNA) to silence the wild-type FMS-like tyrosine kinase 3 (FLT3) gene in acute myeloid leukaemia (AML) cells, in vitro and in vivo. FLT3 siRNA was introduced into the human AML cell line, THP1, and into a THP1 xenograft tumour model in BALB/c nude mice. FLT3 siRNA effectively reduced both the mRNA and the protein levels of FLT3, arrested cells in G₀/G₁ phase, inhibited THP1 cell proliferation and increased apoptosis. Intraperitoneal injection of FLT3 siRNA suppressed tumour growth in BALB/c nude mice. FLT3 siRNA treatment also reduced cyclin D1 and Bcl-2 protein levels, and increased the nuclear level of silencing mediator for retinoic acid and thyroid hormone receptors protein both in vitro and in vivo. These data suggest that FLT3 siRNA is a strong inhibitor of FLT3 expression in vitro and in vivo, and may provide a new therapeutic target for AML.