1α,25Dihydroxyvitamin D3 inhibits the differentiation and bone resorption by osteoclasts generated from Wistar rat bone marrowderived macrophages

Author：

Publisher： Spandidos Publications

E-ISSN： 1792-1015|10|3|1039-1044

ISSN： 1792-0981

Source： Experimental and Therapeutic Medicine, Vol.10, Iss.3, 2015-01, pp. : 1039-1044

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Abstract

The steroid hormone 1α,25dihydroxyvitamin D3 [1α,25(OH)2D3] plays an important role in maintaining a balance in calcium and bone metabolism. To study the effects of 1α,25(OH)2D3 on osteoclast (OC) formation and bone resorption, OC differentiation was induced in bone marrowderived mononuclear cells from Wistar rats with the addition of macrophage colony stimulating factor and receptor activator for nuclear factorκB ligand in vitro. Cells were then treated with 1α,25(OH)2D3 at 109, 108 or 107 mol/l. OCs were identified using tartrateresistant acid phosphatase staining and activity was monitored in the absorption lacunae by scanning electron microscopy. Expression levels of functional proteins associated with bone absorption, namely carbonic anhydrase II, cathepsin K and matrix metalloproteinase9 were evaluated by western blot analysis. The results showed that 1α,25(OH)2D3 inhibited the formation and activation of OCs in a dosedependent manner and downregulated the expression levels of bone absorptionassociated proteins.