Possibility of Progenitor Cell Mobilization during the Hematological Recovery following Peripheral Blood Stem Cell Autograft

Publisher： Karger

E-ISSN： 1421-9662|109|2|57-63

ISSN： 0001-5792

Source： Acta Haematologica, Vol.109, Iss.2, 2003-03, pp. : 57-63

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Abstract

Twenty-four patients with hematological malignancies were studied during recovery following autografting in order to establish the proportion of patients that show CD34+ cell mobilization and the kinetics of mobilized CD34-positive cells. The patients showed a peak in peripheral blood (PB) CD34+ cells after a median of 14 days (range 12–20) following reinfusion. According to the number of circulating CD34+ cells, two groups could be clearly distinguished: 17 patients (group A) with <10 PB CD34+ cells/µl (median 1.2, range 0–5) and 7 patients (group B) with >10 CD34+ cells/µl (median 51, range 13–123). Compared to group A, patients of group B showed a faster hematological reconstitution of both polymorphonuclear leukocytes >500/µl (12 vs. 15 days) and platelets >50,000/µl (12 vs. 17 days). The expression of the β1 integrin CD49d was similar in the two groups of patients, while a lower expression of the β2 integrin CD11a and a greater expression of the L-selectin CD62L were observed in the PB CD34+ cells of group B patients. Both in the PB and in the BM, the number of CFU-GEMM, CFU- GM, CFU-E and BFU-E of group B was significantly greater than that of group A. However, when the clonogenic potential of a single CD34+ cell was evaluated, no major differences in the number of colonies produced per CD34+ cell were found between the two groups.