Publisher: Karger
E-ISSN: 1423-0291|70|2|98-102
ISSN: 1015-2008
Source: Pathobiology, Vol.70, Iss.2, 2002-12, pp. : 98-102
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Abstract
Glomerular IgA deposits were eluted from renal biopsy specimens exhibiting IgA nephropathy (IgAN) by using a combination of citrate buffer and collagenase. Collagenase predigestion of the kidney tissues resulted in increased amounts of IgA eluted by citrate buffer, and the elusion procedure did not attenuate the antigen-binding ability of IgA antibody. When reactivity of the eluted IgA with bacteria components was examined by Western blotting, the most notable reaction was observed for
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