A Novel Quantitative Spectrophotometric Method for the Analysis of Vigabatrin in Pure Form and in Pharmaceutical Formulation

Publisher： Bentham Science Publishers

E-ISSN： 1875-676x|12|4|365-370

ISSN： 1573-4129

Source： Current Pharmaceutical Analysis, Vol.12, Iss.4, 2016-09, pp. : 365-370

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Abstract

Background: Vigabatrin is a variant of the inhibitory neurotransmitter γ;-aminobutyric acid(GABA). It reduces seizure activities by inhibiting irreversibly GABA transaminase and increasingGABA concentrations.Objective: This study presents simple, sensitive, extraction-free, and cost-efficient estimation of vigabatrinusing a spectrophotometric method in pharmaceutical formulation.Method: The method relies on the development of water-soluble, blue-violet colored complexes of vigabatrinand ninhydrin in a phosphate buffer of pH 7.4 with λ;_max at 565 nm. Linearity, accuracy, precision,and specificity were performed as validation parameters for this method.Results: Assessment of different analytical factors was carried out, and statistical analysis was performedto validate the results. The findings showed a linear progression in absorbance when the concentration ofvigabatrin was increased. The correlation coefficient value (r²) of 0.9981 was observed. The operationsfollowed the Beer’s law in the span of 40-200 µ;gmL^-1 with a molar absorptivity of 5.16x10³ Lmol^–1cm^–1.Recovery values in the marketed formulation of the assay method indicated no interference from thecommon additives and excipients. The limits of detection and quantification were found to be 6.0 and 40µ;gmL^-1, respectively.Conclusion: The presented method has demonstrated to be delicate, reliable, explicit, and intuitive thatcan prove beneficial for regular laboratory examination of vigabatrin in pharmaceutical dosage forms.';