Determination of Imatinib and CGP74588 in Rat Plasma by HPLC and Its Application to a Pharmacokinetic Study

E-ISSN： 1875-676x|14|2|133-138

ISSN： 1573-4129

Source： Current Pharmaceutical Analysis, Vol.14, Iss.2, 2018-01, pp. : 133-138

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Abstract

Objective: A simple, reliable and specific method of HPLC which was performed on a reversed-phase Agilent SB column was developed for the determination of imatinib and CGP74588.Method: The mobile phase comprised water-acetonitrile-0.1% trifluoroacetic acid (59:21:20, v/v/v)flowed at the rate of 1 mL/min at 40°C. The detection wavelength was 278nm.Results: Sensitivity has been illustrated in this method, and a good linear relationship was observedbetween 0.1-20.00 µg/mL for imatinib with r2=0.9994 and 0.01-2.00 µg/mL for CGP74588 withr2=0.9998 in rat plasma. The relative recoveries of imatinib and CGP74588 were all more than 93%,whilst the absolute recoveries of imatinib and CGP74588 were all more than 75%. Both intra- andinter-day precisions were all less than 4.5% for imatinib and 4.74% for GCP74588. The limits of quantificationof these analytic methods were 0.1 µg/mL for imatinib and 0.01 µg/mL for CGP74588.Conclusion: The method was suitable for studying the pharmacokinetic parameters of imatinib andGCP74588 and routine analysis.