Extracellular Synthesis of Selenium Nanoparticles from an Actinomycetes Streptomyces griseoruber and Evaluation of its Cytotoxicity on HT-29 Cell Line

Publisher： Bentham Science Publishers

E-ISSN： 2211-7393|6|1|61-68

ISSN： 2211-7385

Source： Pharmaceutical Nanotechnology, Vol.6, Iss.1, 2018-04, pp. : 61-68

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Abstract

Background: Selenium nanoparticles (SeNPs) have gained significant importance becauseof its bioavailability, least toxicity, its interaction with proteins and its biocompatibility.Objective: In the present study, the extracellular synthesis of SeNPs was carried out by using culturesupernatant of Streptomyces griseoruber, an Actinomycetes member isolated from the soil and cytotoxicitywas tested on HT-29 cell line.Methods: Culture supernatant was mixed with 1mM sodium selenite for the biosynthesis of SeNPs.Characterisation of the synthesised SeNPs was done by UV-Visible spectrophotometer, FTIR, XRD,DLS and HR-TEM. The cytotoxicity of nanoparticles on HT-29 cell line was studied by MTT assayand with different staining procedure.Results: Bioreduction of SeNPs was confirmed by UV-Visible spectrophotometer that showed thepeak at 575 nm. Size and distribution of the biosynthesised SeNPs were analysed by HR-TEM thatshowed the formation of particle size in the range of 100-250nm. The synthesised SeNPs showed goodcytotoxic activity against HT-29 cell line with 40.5%, 33% and 23.7% of cell viability at 2μg/ ml,4μg/ml and 30μg/ml concentration respectively.Conclusion: The present study reports the simple and eco-friendly synthesis of SeNPs that showedgood cytotoxic activity against HT-29 cell line suggesting that biogenic SeNPs could be a potentialchemotherapeutic agent.