Capillary Electrophoresis Interfaced with a Mass Spectrometer (CE-MS): Technical Considerations and Applicability for Biomarker Studies in Animals

Publisher: Bentham Science Publishers

E-ISSN: 1875-5550|15|1|23-35

ISSN: 1389-2037

Source: Current Protein and Peptide Science, Vol.15, Iss.1, 2014-03, pp. : 23-35

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Abstract

Proteomics is a growing field that has the potential to be applied to many biology-related disciplines. However,the study of the proteome has proven to be very challenging due to its high level of complexity when compared to genomeand transcriptome data. In order to analyse this level of complexity, high resolution separation of peptides/proteins areneeded together with high resolution analysers. Currently, liquid chromatography and capillary electrophoresis (CE) arethe two most widely used separation techniques that can be coupled on-line with a mass spectrometer (MS). In CE, proteins/peptides are separated according to their size, charge and shape leading to high resolving power. Although furtherprogress in the area of sensitivity, throughput and proteome coverage are expected, MS-based proteomics have developedto a level at which they are habitually applied to study a wide range of biological questions. The aim of this review is topresent CE-MS as a proteomic analytical platform for biomarker research that could be used in farm animal and veterinarystudies. This is a MS-analytical platform that has been widely used for biomarker research in the biomedical field but itsapplication in animal proteomic studies is relatively novel. The review will focus on introducing the CE-MS platform andthe primary considerations for its application to biomarker research. Furthermore, current applications but more importantlypotential application in the field of farm animals and veterinary science will be presented and discussed.