Publisher： John Wiley & Sons Inc
E-ISSN： 1744-3091|66|11|1470-1472
ISSN： 1744-3091
Source： Acta Crystallographica Section F, Vol.66, Iss.11, 2010-11, pp. : 1470-1472
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Abstract
Dissimilatory sulfite reductase (Dsr) plays an important role in sulfate respiration in many sulfate‐reducing bacteria. Dsr from Desulfovibrio vulgaris Miyazaki F has been purified and crystallized at 277 K using the sitting‐drop vapour‐diffusion method with PEG 3350 and potassium thiocyanate as precipitants. A data set was collected to 3.7 Å resolution from a single crystal at 100 K using synchrotron radiation. The Dsr crystal belonged to space group P41212, with unit‐cell parameters a = b = 163.26, c = 435.32 Å. The crystal structure of Dsr was determined by the molecular‐replacement method based on the three‐dimensional structure of Dsr from D. vulgaris Hildenborough. The crystal contained three α2β2γ2 units per asymmetric unit, with a Matthews coefficient (VM) of 2.35 Å3 Da−1; the solvent content was estimated to be 47.7%.
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