Purification, crystallization and preliminary X‐ray crystallographic analysis of MIL, a glycosylated jacalin‐related lectin from mulberry (Morus indica) latex

Publisher: John Wiley & Sons Inc

E-ISSN: 1744-3091|67|5|608-612

ISSN: 1744-3091

Source: Acta Crystallographica Section F, Vol.67, Iss.5, 2011-05, pp. : 608-612

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Abstract

A quantitatively major protein has been purified from the latex of Morus indica. The purified previously uncharacterized protein, M. indica lectin (MIL), was further shown to be a glycosylated tetramer and belongs to the family of jacalin‐related lectins. Crystallization of MIL was also accomplished and the tetragonal crystals diffracted synchrotron X‐rays to a resolution of 2.8 Å.