Publisher： John Wiley & Sons Inc
E-ISSN： 1744-3091|66|3|282-285
ISSN： 1744-3091
Source： Acta Crystallographica Section F, Vol.66, Iss.3, 2010-03, pp. : 282-285
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
A thermostable manganese(II)‐dependent 2,3‐dihydroxybiphenyl‐1,2‐dioxygenase derived from Bacillus sp. JF8 was crystallized. The initial screening for crystallization was performed by the sitting‐drop vapour‐diffusion method using a crystallization robot, resulting in the growth of two crystal forms. The first crystal belonged to space group P1, with unit‐cell parameters a = 62.7, b = 71.4, c = 93.6 Å, α = 71.2, β = 81.0, γ = 64.0°, and diffracted to 1.3 Å resolution. The second crystal belonged to space group I222, with unit‐cell parameters a = 74.2, b = 90.8, c = 104.3 Å, and diffracted to 1.3 Å resolution. Molecular‐replacement trials using homoprotocatechuate 2,3‐dioxygenase from Arthrobacter globiformis (28% amino‐acid sequence identity) as a search model provided a satisfactory solution for both crystal forms.
Related content
Access to resources
Recommend
