Publisher： John Wiley & Sons Inc
E-ISSN： 1744-3091|64|9|858-862
ISSN： 1744-3091
Source： Acta Crystallographica Section F, Vol.64, Iss.9, 2008-09, pp. : 858-862
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Abstract
Succinate:ubiquinone oxidoreductase (SQR) was solubilized and purified from Escherichia coli inner membranes using several different detergents. The number of phospholipid molecules bound to the SQR molecule varied greatly depending on the detergent combination that was used for the solubilization and purification. Crystallization conditions were screened for SQR that had been solubilized and purified using 2.5%(w/v) sucrose monolaurate and 0.5%(w/v) Lubrol PX, respectively, and two different crystal forms were obtained in the presence of detergent mixtures composed of n‐alkyl‐oligoethylene glycol monoether and n‐alkyl‐maltoside. Crystallization took place before detergent phase separation occurred and the type of detergent mixture affected the crystal form.
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