Publisher： John Wiley & Sons Inc
E-ISSN： 1744-3091|65|11|1187-1189
ISSN： 1744-3091
Source： Acta Crystallographica Section F, Vol.65, Iss.11, 2009-11, pp. : 1187-1189
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Abstract
PASTA subunits (∼70 amino acids) are specific to bacterial serine/threonine kinases and to penicillin‐binding proteins (PBPs) and are involved in the synthesis of peptidoglycan. The human pathogen Staphylococcus aureus contains a serine/threonine kinase, Stk1, which plays a major role in virulence. A recombinant His‐tagged portion of the extracellular domain of Stk1 containing three PASTA subunits has been crystallized using zinc sulfate as a crystallizing agent. The crystals belonged to the tetragonal space group P4122, with unit‐cell parameters a = 68.0, b = 68.0, c = 158.1 Å. Structure determination by the MAD method is now in progress.
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