Purification, crystallization and preliminary X‐ray analysis of two hydrogen sulfide‐producing enzymes from Fusobacterium nucleatum

Publisher: John Wiley & Sons Inc

E-ISSN: 1744-3091|68|12|1507-1510

ISSN: 1744-3091

Source: Acta Crystallographica Section F, Vol.68, Iss.12, 2012-12, pp. : 1507-1510

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Abstract

Hydrogen sulfide produced by oral bacteria is responsible for oral malodour. Two homologous hydrogen sulfide‐producing enzymes, Fn1220 and Cdl, from Fusobacterium nucleatum (which actively produces hydrogen sulfide) were overproduced, purified and crystallized. X‐ray diffraction data were collected from the crystals using a synchrotron‐radiation source. The Fn1220 crystal belonged to tetragonal space group P41212 or P43212 (unit‐cell parameters a = b = 116.8, c = 99.2 Å) and the Cdl crystal belonged to monoclinic space group P21 (unit‐cell parameters a = 84.9, b = 70.9, c = 87.6 Å, β = 90.3°).