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Crystallization and preliminary X‐ray diffraction analysis of cyclic imide hydrolase (CIH) from Pseudomonas putida YZ‐26

Publisher: John Wiley & Sons Inc

E-ISSN: 1744-3091|67|4|521-523

ISSN: 1744-3091

Source: Acta Crystallographica Section F, Vol.67, Iss.4, 2011-04, pp. : 521-523

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Abstract

A recombinant form of cyclic imide hydrolase from Pseudomonas putida YZ‐26 has been crystallized by the hanging‐drop method. X‐ray diffraction data were collected to 1.9 Å resolution. The crystals belonged to the orthorhombic space group C2221, with unit‐cell parameters a = 111.91, b = 176.04, c = 176.06 Å. Assuming the presence of four molecules in the asymmetric unit gives a VM value of 3.10 Å3 Da−1 and a solvent content of 60.31%.