Crystallization and preliminary X‐ray diffraction analysis of a putative two‐domain‐type laccase from a metagenome

Publisher： John Wiley & Sons Inc

E-ISSN： 1744-3091|65|3|264-266

ISSN： 1744-3091

Source： Acta Crystallographica Section F, Vol.65, Iss.3, 2009-03, pp. : 264-266

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Abstract

A putative two‐domain‐type laccase retrieved from a metagenome was successfully crystallized using the sitting‐drop vapour‐diffusion method. Data were collected to a resolution of 1.7 Å at 100 K using synchrotron radiation. The crystal belonged to space group P212121, with unit‐cell parameters a = 74.67, b = 100.95, c = 124.11 Å. The self‐rotation function showed the presence of a noncrystallographic threefold axis in the structure. The presence of one trimer in the asymmetric unit yielded a Matthews coefficient (VM) of 2.05 Å3 Da−1 and a solvent content of 40%.

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