Expression, purification and preliminary crystallographic analysis of sucrose phosphate synthase (SPS) from Halothermothrix orenii

Publisher: John Wiley & Sons Inc

E-ISSN: 1744-3091|61|1|116-117

ISSN: 1744-3091

Source: Acta Crystallographica Section F, Vol.61, Iss.1, 2005-01, pp. : 116-117

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Abstract

This is the first report of the crystallization of a sucrose phosphate synthase (SPS; EC 2.4.1.14). It also constitutes the first study of a sucrose phosphate synthase from a non‐photosynthetic thermohalophilic anaerobic bacterium, Halothermothrix orenii. The purified recombinant spsA protein has been crystallized in the monoclinic space group C2, with unit‐cell parameters a = 154.2, b = 47.9, c = 72.3 Å, β = 103.16°, using the hanging‐drop vapour‐diffusion method. The crystal diffracts X‐rays to a resolution limit of 3.01 Å. Heavy‐metal and halide‐soaking trials are currently in progress to solve the structure.

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