Publisher： John Wiley & Sons Inc
E-ISSN： 1744-3091|62|3|235-237
ISSN： 1744-3091
Source： Acta Crystallographica Section F, Vol.62, Iss.3, 2006-03, pp. : 235-237
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Abstract
A lectin from Cymbosema roseum seeds (CRL) was purified, characterized and crystallized. The best crystals grew in a month and were obtained by the vapour‐diffusion method using a precipitant solution consisting of 0.1 M Tris–HCl pH 7.8, 8%(w/v) PEG 3350 and 0.2 M proline at a constant temperature of 293 K. A data set was collected to 1.77 Å resolution at a synchrotron‐radiation source. CRL crystals are orthorhombic, belonging to space group P212121. Crystallographic refinement and full amino‐acid sequence determination are in progress.
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