Molecular insights gained from covalently tethering cGMP to the ligand-binding sites of retinal rod cGMP-gated channels

Publisher: Cambridge University Press

E-ISSN: 1469-1825|18|3|471-472

ISSN: 0140-525x

Source: Behavioral and Brain Sciences, Vol.18, Iss.3, 1995-09, pp. : 471-472

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Abstract

A photoaffinity analog of cGMP has been used to biochemically identify a new ligand-binding subunit of the retinal rod cGMP-activated ion channel, as well as amino acids in contact with cGMP in the original subunit. Covalent tethering of this probe to channels in excised menbrane patches has revealed a functional heteogeneity in the ligand-binding sites that may arise from the two biochemically identified subunits.