Colonisation of Glossina morsitans morsitans Westw. (Diptera, Glossinidae) in the laboratory using in vitro feeding techniques

Publisher： Cambridge University Press

E-ISSN： 1475-2670|65|4|631-642

ISSN： 0007-4853

Source： Bulletin of Entomological Research, Vol.65, Iss.4, 1976-02, pp. : 631-642

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Abstract

Fresh defibrinated bovine blood was superior to either haemolysed or freeze-dried defibrinated bovine blood when fed in vitro to female Glossina morsitans morsitans Westw. The presence of adenosine-5′-triphosphate (ATP) or other additives did not improve reproductive performance. The flies were able to compensate for considerable dilution of the blood with both serum and saline. Both parental and F₁ female size affected the weight of the F₂ puparia produced. The feeding of females on rabbits' ears on one day and in vitro on five days per week prevented any deterioration in performance over successive generations compared with a purely in vitro regime. A colony was established using this technique and results over a 13-month period were compared with those from a colony fed without rabbit supplement and another fed on rabbits only. The reproductive performance of females in the all-membrane-fed colony deteriorated from generation to generation, while those in the rabbit supplement colony performed similarly to those of the rabbit-fed colony except that survival was better but fecundity was poorer and the puparia produced were smaller.