Mammary metabolism in lactating sows: arteriovenous differences of milk precursors and the mammary metabolism of [14C]glucose and [14C]acetate

Publisher: Cambridge University Press

E-ISSN: 1475-2662|23|2|319-333

ISSN: 0007-1145

Source: British Journal of Nutrition, Vol.23, Iss.2, 1969-06, pp. : 319-333

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Abstract

1. The following techniques, which have been applied successfully to goats, were used to study mammary metabolism in lactating sows:(I) measurements of mammary arteriovenous (A-V) differences in milk precursors in the conscious undisturbed animal (five sows); (2) continuous intravenous infusion of [U-14C]glucose with concomitant arterial and mammary venous blood sampling for measurement of mammary blood flow and specific radioactivity of glucose and CO, (one sow); (3) perfusion of the isolated gland in vitro (eight glands from four sows), with the inclusion of [U-14C]glucose (two glands) and [U-14C]acetate(two glands) in the substrate mixture.