Cloning, Expression, Purification and Crystallization of the PR Domain of Human Retinoblastoma Protein-Binding Zinc Finger Protein 1 (RIZ1)

Author： Sun Wanpeng Geyer C. Ronald Yang Jian

Publisher： MDPI

E-ISSN： 1422-0067|9|6|943-950

ISSN： 1422-0067

Source： International Journal of Molecular Sciences, Vol.9, Iss.6, 2008-06, pp. : 943-950

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Abstract

Through alternative promoter usage, human retinoblastoma protein-interacting zinc finger gene RIZ encodes two different protein products, RIZ1 and RIZ2, which have been identified to be a tumor suppressor and a proto-oncoprotein, respectively. Structurally, the two protein products share the same amino acid sequences except that RIZ2 lacks an N-terminal PR domain with methyltransferase activity. Previous studies have shown that over-expression of RIZ2 is usually associated with depressed RIZ1 expression in different human cancers. It is generally believed that RIZ1 and RIZ2 regulate normal cell division and function using a “Yin-Yang” fashion and the PR domain is responsible for the tumor suppressing activity of RIZ1. In order to better understand the biological functions of the PR domain by determining its three-dimensional crystal structure, we expressed, purified and crystallized a construct of the PR domain (amino acid residues 13–190) in this study. The maximum size of the needle-shaped crystals was approximately 0.20 × 0.01 × 0.01 mm.