DNA Extraction Systematics for Spectroscopic Studies

Author: Palma Bianca Fogazza   Ferrari Amanda Borges   Bitar Renata Andrade   Cardoso Maria Angélica Gargione   Martin Airton A.   da Silva Martinho Herculano  

Publisher: MDPI

E-ISSN: 1424-8220|8|6|3624-3632

ISSN: 1424-8220

Source: Sensors, Vol.8, Iss.6, 2008-06, pp. : 3624-3632

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Abstract

Study of genetic material allows the comprehension the origin of the many biochemical changes that follow diseases, like cancer, promoting the development of early preventive inquiry and more efficient individual treatments. Raman spectroscopy can be an important tool in DNA study, since it allows probe molecular vibrations of genetic material in a fast way. The present work established a systematic way for extract DNA in suitable concentrations and structural integrity allowing studies by Raman spectroscopy or other spectroscopic technique, including bio-analytical sensors for probing genetic alterations.