Antiviral Activity and Mechanism of Action of Novel Thiourea Containing Chiral Phosphonate on Tobacco Mosaic Virus

Author： Fan Huitao   Song Baoan   Bhadury Pinaki S.   Jin Linhong   Hu Deyu   Yang Song  

Publisher： MDPI

E-ISSN： 1422-0067|12|7|4522-4535

ISSN： 1422-0067

Source： International Journal of Molecular Sciences, Vol.12, Iss.7, 2011-07, pp. : 4522-4535

Access to resources Favorite

Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.

Previous Menu Next

Abstract

Using half-leaf method O,O′-diisopropyl (3-(L-1-(benzylamino)-1-oxo-3- phenylpropan-2-yl)thioureido)(phenyl)methyl phosphonate (2009104) was studied for its activity on tobacco mosaic virus (TMV). It showed good curative activity in vivo and the curative activity at 500 μg/mL was found to be 53.3%. In vivo treatment with the control agent Ningnanmycin at 500 μg/mL resulted in 51.2% inhibition and curative inhibition rates respectively. Dot-ELISA test was employed to verify the efficacy of activity of compound 200910 for anti-TMV activity. The mechanism of action of compound 2009104 to resist TMV was also studied. The results showed that the resistance enzymes PAL, POD, SOD activity and chlorophyll content after TMV inoculation K326 (Nicotiana tabacum K326) of tobacco plants followed by treatment with compound 2009104 were significantly enhanced. The study of the effect of compound 2009104 on TMV capsid protein (CP) showed that it inhibited the polymerization process of TMV-CP in vitro.

Related content

Antiviral Activity of a Novel Compound CW-33 against Japanese Encephalitis Virus through Inhibiting Intracellular Calcium Overload

By Huang Su-Hua   Lien Jin-Cherng   Chen Chao-Jung   Liu Yu-Ching   Wang Ching-Ying   Ping Chia-Fong   Lin Yu-Fong   Huang An-Cheng   Lin Cheng-Wen  

International Journal of Molecular Sciences, Vol. 17, Iss. 9, 2016-08 ,pp. : 1386-1386 (1)

MDPI

Access to resources Recommend Favorite

Nanoparticle Encapsidation of Flock House Virus by Auto Assembly of Tobacco Mosaic Virus Coat Protein

By Maharaj Payal D.   Mallajosyula Jyothi K.   Lee Gloria   Thi Phillip   Zhou Yiyang   Kearney Christopher M.   McCormick Alison A.  

International Journal of Molecular Sciences, Vol. 15, Iss. 10, 2014-10 ,pp. : 18540-18556 (17)

MDPI

Access to resources Recommend Favorite

Antiviral Activity of Baicalein and Quercetin against the Japanese Encephalitis Virus

By Johari Jefree   Kianmehr Aynaz   Mustafa Mohd Rais   Abubakar Sazaly   Zandi Keivan  

International Journal of Molecular Sciences, Vol. 13, Iss. 12, 2012-12 ,pp. : 16785-16795 (11)

MDPI

Access to resources Recommend Favorite

New Strategies and Methods to Study Interactions between Tobacco Mosaic Virus Coat Protein and Its Inhibitors

By Li Xiangyang   Chen Zhuo   Jin Linhong   Hu Deyu   Yang Song  

International Journal of Molecular Sciences, Vol. 17, Iss. 3, 2016-02 ,pp. : 252-252 (1)

MDPI

Access to resources Recommend Favorite

Repositioning of Thiourea-Containing Drugs as Tyrosinase Inhibitors

By Choi Joonhyeok   Jee Jun-Goo  

International Journal of Molecular Sciences, Vol. 16, Iss. 12, 2015-12 ,pp. : 28534-28548 (15)

MDPI

Access to resources Recommend Favorite