Resveratrol Protects against 2-Bromopropane-Induced Apoptosis and Disruption of Embryonic Development in Blastocysts

Publisher： MDPI

E-ISSN： 1422-0067|12|8|4991-5010

ISSN： 1422-0067

Source： International Journal of Molecular Sciences, Vol.12, Iss.8, 2011-08, pp. : 4991-5010

Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.

Previous Menu Next

Abstract

2-Bromopropane (2-BP) is used as an alternative to ozone-depleting cleaning solvents. Previously, we reported that 2-BP has cytotoxic effects on mouse blastocysts and is associated with defects in subsequent development. In the present work, we show that 2-BP induces apoptosis in the inner cell mass of mouse blastocysts, and inhibits cell proliferation. Both effects are suppressed by resveratrol, a grape-derived phytoalexin with known antioxidant and anti-inflammatory properties. 2-BP-treated blastocysts displayed lower levels of implantation (compared to controls) when plated on culture dishes in vitro, and a reduced ability to proceed to later stages of embryonic development. Pretreatment with resveratrol prevented 2-BP-induced disruption of embryonic development, both in vitro and in vivo. Further investigation of these processes revealed that 2-BP directly promotes ROS generation, loss of mitochondrial membrane potential (MMP), and activation of caspase-3, whereas resveratrol effectively blocks 2-BP-induced ROS production and the accompanying apoptotic biochemical changes. Our results collectively imply that 2-BP triggers the mitochondrion-dependent apoptotic pathway via ROS generation, and the antioxidant activity of resveratrol prevents 2-BP-induced toxicity.

Related content

Cytotoxic Effects of 2-Bromopropane on Embryonic Development in Mouse Blastocysts

By Chan Wen-Hsiung

International Journal of Molecular Sciences, Vol. 11, Iss. 2, 2010-02 ,pp. : 731-744 (14)

MDPI

Access to resources Recommend Favorite

Cytotoxic Effects of Dillapiole on Embryonic Development of Mouse Blastocysts in Vitro and in Vivo

By Chan Wen-Hsiung

International Journal of Molecular Sciences, Vol. 15, Iss. 6, 2014-06 ,pp. : 10751-10765 (15)

MDPI

Access to resources Recommend Favorite

Resveratrol Protects against Helicobacter pylori-Associated Gastritis by Combating Oxidative Stress

By Zhang Xiaolin Jiang Anmin Qi Banghua Ma Zhongyou Xiong Youyi Dou Jinfeng Wang Jianfei

International Journal of Molecular Sciences, Vol. 16, Iss. 11, 2015-11 ,pp. : 27757-27769 (13)

MDPI

Access to resources Recommend Favorite

Urotensin II Protects Cardiomyocytes from Apoptosis Induced by Oxidative Stress through the CSE/H₂S Pathway

By Gong Hui Chen Zhidan Zhang Xiaoyi Li Yang Zhang Jie Chen Ying Ding Yingjiong Zhang Guoping Yang Chunjie Zhu Yichun Zou Yunzeng

International Journal of Molecular Sciences, Vol. 16, Iss. 6, 2015-06 ,pp. : 12482-12498 (17)

MDPI

Access to resources Recommend Favorite

Selenoprotein R Protects Human Lens Epithelial Cells against d-Galactose-Induced Apoptosis by Regulating Oxidative Stress and Endoplasmic Reticulum Stress

By Dai Jie Liu Hongmei Zhou Jun Huang Kaixun

International Journal of Molecular Sciences, Vol. 17, Iss. 2, 2016-02 ,pp. : 231-231 (1)

MDPI

Access to resources Recommend Favorite