

Author: Opstal-van Winden Annemieke W. J. Rodenburg Wendy Pennings Jeroen L. A. van Oostrom Conny T. M. Beijnen Jos H. Peeters Petra H.M. van Gils Carla H. de Vries Annemieke
Publisher: MDPI
E-ISSN: 1422-0067|13|10|13587-13604
ISSN: 1422-0067
Source: International Journal of Molecular Sciences, Vol.13, Iss.10, 2012-10, pp. : 13587-13604
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
This study investigates whether a set of ten potential breast cancer serum biomarkers and cancer antigens (osteopontin (OPN), haptoglobin, cancer antigen 15-3 (CA15-3), carcinoembryonic antigen (CEA), cancer antigen 125 (CA-125), prolactin, cancer antigen 19-9 (CA19-9), α-fetoprotein (AFP), leptin and migration inhibitory factor (MIF)) can predict early stage breast cancer in samples collected before clinical diagnosis (phase III samples). We performed a nested case-control study within the Prospect-EPIC (European Prospective Investigation into Cancer and nutrition) cohort. We examined to what extent the biomarker panel could discriminate between 68 women diagnosed with breast cancer up to three years after enrollment and 68 matched healthy controls (all 56–64 years at baseline). Using a quantitative bead-based multiplexed assay, we determined protein concentrations in serum samples collected at enrollment. Principal Component Analysis (PCA) and Random Forest (RF) analysis revealed that on the basis of all ten proteins, early cases could not be separated from controls. When we combined serum protein concentrations and subject characteristics related to breast cancer risk in the RF analysis, this did not result in classification accuracy scores that could correctly classify the samples (sensitivity: 50%, specificity: 50%). Our findings indicate that this panel of selected tumor markers cannot be used for diagnosis of early breast cancer.
Related content


By Li Nan Liu Ye Hao Zhuo Zhang Shoufeng Hu Rongliang Li Jiping
International Journal of Molecular Sciences, Vol. 15, Iss. 4, 2014-03 ,pp. :






MicroRNAs as Diagnostic Biomarkers in Gastric Cancer
By Yin Ying Li Jun Chen Shujie Zhou Tianhua Si Jianmin
International Journal of Molecular Sciences, Vol. 13, Iss. 10, 2012-10 ,pp. :


By Berghuis A. M. Sofie Koffijberg Hendrik Prakash Jai Terstappen Leon W. M. M. IJzerman Maarten J.
International Journal of Molecular Sciences, Vol. 18, Iss. 2, 2017-02 ,pp. :