An Ultrasensitive Chemiluminescence Biosensor for Carcinoembryonic Antigen Based on Autocatalytic Enlargement of Immunogold Nanoprobes

Author： Hao Minjia Ma Zhanfang

Publisher： MDPI

E-ISSN： 1424-8220|12|12|17320-17329

ISSN： 1424-8220

Source： Sensors, Vol.12, Iss.12, 2012-12, pp. : 17320-17329

Access to resources Favorite

Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.

Previous Menu Next

Abstract

A sensitive flow injection chemiluminescence assay for carcinoembryonic antigen (CEA) detection based on signal amplification with gold nanoparticles (NPs) is reported in the present work. The sandwich system of CEA/anti-CEA/goat-anti-mouse IgG functionalized Au nanoparticles was used as the sensing platform. In order to improve detection sensitivity, a further gold enlargement step was developed based on the autocatalytic Au deposition of gold nanoprobes via the reduction of AuCl₄⁻ to Au⁰ on their surface in the presence of NH₂OH·HCl. AuCl₄⁻, which is a soluble product of gold nanoprobes, served as an analyte in the CL reaction for the indirect measurement of CEA. Under optimized conditions, the CL intensity of the system was linearly related to the logarithm of CEA concentration in the range of 100 pg·mL⁻¹ to 1,000 ng·mL⁻¹, with a detection limit of 20 pg·mL⁻¹.