Analysis and Design of Stimulus Response Curves of E. coli

Author： Kremling Andreas Goehler Anna Jahreis Knut Nees Markus Auerbach Benedikt Schmidt-Heck Wolfgang Kökpinar Öznur Geffers Robert Rinas Ursula Bettenbrock Katja

Publisher： MDPI

E-ISSN： 2218-1989|2|4|844-871

ISSN： 2218-1989

Source： Metabolites, Vol.2, Iss.4, 2012-11, pp. : 844-871

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Abstract

Metabolism and signalling are tightly coupled in bacteria. Combining several theoretical approaches, a core model is presented that describes transcriptional and allosteric control of glycolysis in Escherichia coli. Experimental data based on microarrays, signaling components and extracellular metabolites are used to estimate kinetic parameters. A newly designed strain was used that adjusts the incoming glucose flux into the system and allows a kinetic analysis. Based on the results, prediction for intracelluar metabolite concentrations over a broad range of the growth rate could be performed and compared with data from literature.