Author: Li Jinping Chen Haibin Mariani Andrea Chen Dong Klatt Edward Podratz Karl Drapkin Ronny Broaddus Russell Dowdy Sean Jiang Shi-Wen
Publisher: MDPI
E-ISSN: 1422-0067|14|3|6026-6043
ISSN: 1422-0067
Source: International Journal of Molecular Sciences, Vol.14, Iss.3, 2013-03, pp. : 6026-6043
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Abstract
HE4, also known as WFDC2, is a useful biomarker for ovarian cancer when either used alone or in combination with CA125. HE4 is also overexpressed in endometrial cancer (EC), but its function in cancer cells is not clear. In this study, we investigate the role of HE4 in EC progression. An HE4-overexpression system was established by cloning the HE4 prototypic mRNA variant (HE4-V0) into a eukaryotic expression vector. Following transfection, stable clones in two EC cell lines were selected. The effects of HE4 overexpression on cell growth and function were measured with the use of cell proliferation assay, matrigel invasion, and soft agar gel colony formation assays. HE4-induced cancer cell proliferation
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