Magnetic Drug Targeting Reduces the Chemotherapeutic Burden on Circulating Leukocytes

Author： Janko Christina Dürr Stephan Munoz Luis E. Lyer Stefan Chaurio Ricardo Tietze Rainer von Löhneysen Sarah Schorn Christine Herrmann Martin Alexiou Christoph

Publisher： MDPI

E-ISSN： 1422-0067|14|4|7341-7355

ISSN： 1422-0067

Source： International Journal of Molecular Sciences, Vol.14, Iss.4, 2013-04, pp. : 7341-7355

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Abstract

Magnetic drug targeting (MDT) improves the integrity of healthy tissues and cells during treatment with cytotoxic drugs. An anticancer drug is bound to superparamagnetic iron oxide nanoparticles (SPION), injected into the vascular supply of the tumor and directed into the tumor by means of an external magnetic field. In this study, we investigated the impact of SPION, mitoxantrone (MTO) and SPION^MTO on cell viability in vitro and the nonspecific uptake of MTO into circulating leukocytes in vivo. MDT was compared with conventional chemotherapy. MTO uptake and the impact on cell viability were assessed by flow cytometry in a Jurkat cell culture. In order to analyze MTO loading of circulating leukocytes in vivo, we treated tumor-bearing rabbits with MDT and conventional chemotherapy. In vitro experiments showed a dose-dependent MTO uptake and reduction in the viability and proliferation of Jurkat cells. MTO and SPION^MTO showed similar cytotoxic activity. Non-loaded SPION did not have any effect on cell viability in the concentrations tested. Compared with systemic administration in vivo, MDT employing SPION^MTO significantly decreased the chemotherapeutic load in circulating leukocytes. We demonstrated that MDT spares the immune system in comparison with conventional chemotherapy.