Author： Di Maïo Isabelle L. Barbier Pascale Allegro Diane Brault Cédric Peyrot Vincent
Publisher： MDPI
E-ISSN： 1422-0067|15|8|14697-14714
ISSN： 1422-0067
Source： International Journal of Molecular Sciences, Vol.15, Iss.8, 2014-08, pp. : 14697-14714
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Abstract
The interaction between the microtubule associated protein, tau and the microtubules is investigated. A fluorescence resonance energy transfer (FRET) assay was used to determine the distance separating tau to the microtubule wall, as well as the binding parameters of the interaction. By using microtubules stabilized with Flutax-2 as donor and tau labeled with rhodamine as acceptor, a donor-to-acceptor distance of 54 ± 1 Å was found. A molecular model is proposed in which Flutax-2 is directly accessible to tau-rhodamine molecules for energy transfer. By titration, we calculated the stoichiometric dissociation constant to be equal to 1.0 ± 0.5 µM. The influence of the
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