Author： Jeziorowska Dorota Fontaine Vincent Jouve Charlène Villard Eric Dussaud Sébastien Akbar David Letang Valérie Cervello Pauline Itier Jean-Michiel Pruniaux Marie-Pierre Hulot Jean-Sébastien
Publisher： MDPI
E-ISSN： 1422-0067|18|6|1173-1173
ISSN： 1422-0067
Source： International Journal of Molecular Sciences, Vol.18, Iss.6, 2017-06, pp. : 1173-1173
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Abstract
Human induced pluripotent stem cells (iPSCs) represent a powerful human model to study cardiac disease in vitro, notably channelopathies and sarcomeric cardiomyopathies. Different protocols for cardiac differentiation of iPSCs have been proposed either based on embroid body formation (3D) or, more recently, on monolayer culture (2D). We performed a direct comparison of the characteristics of the derived cardiomyocytes (iPSC-CMs) on day 27 ± 2 of differentiation between 3D and 2D differentiation protocols with two different Wnt-inhibitors were compared: IWR1 (inhibitor of Wnt response) or IWP2 (inhibitor of Wnt production). We firstly found that the level of Troponin T (
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