The Implication of Substance P in the Development of Tendinopathy: A Case Control Study

Author： Han Soo-Hong Choi Wonchul Song Jiye Kim Jaehee Lee Seungyong Choi Youngrak Byun Seong-Eun Ahn Taekeun Ahn Heejung Ding Catherine Baik Lloyd Ward Spencer Ting Kang Lee Soonchul

Publisher： MDPI

E-ISSN： 1422-0067|18|6|1241-1241

ISSN： 1422-0067

Source： International Journal of Molecular Sciences, Vol.18, Iss.6, 2017-06, pp. : 1241-1241

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Abstract

It was reported that substance P had beneficial effects in the healing of acute tendon injury. However, the relationship between substance P and degenerative tendinopathy development remains unclear. The purpose of this study was to determine the role of substance P in the pathogenesis of tendinopathy. Healthy and tendinopathy tendon were harvested from human and tenocytes were cultured individually. The expression levels of genes associated with tendinopathy were compared. Next, substance P was exogenously administered to the healthy tenocyte and the effect was evaluated. The results showed that tendinopathy tenocytes had higher levels of COL3A1, MMP1, COX2, SCX, ACTA2, and substance P gene expression compared to healthy tenocytes. Next, substance P treatment on the healthy tenocyte displayed similar changes to that of the tendinopathy tenocytes. These differences between the two groups were also determined by Western blot. Additionally, cells with substance P had the tendinopathy change morphologically although cellular proliferation was significantly higher compared to that of the control group. In conclusion, substance P enhanced cellular proliferation, but concomitantly increased immature collagen (type 3 collagen). Substance P plays a crucial role in tendinopathy development and could be a future therapeutic target for treatment.