Resveratrol amplifies BMP4stimulated osteoprotegerin synthesis via p38 MAP kinase in osteoblasts

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E-ISSN： 1791-3004|12|3|3849-3854

ISSN： 1791-2997

Source： Molecular Medicine Reports, Vol.12, Iss.3, 2015-01, pp. : 3849-3854

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Abstract

Resveratrol is a naturally occurring polyphenol that possesses healthrelated properties, and is predominantly found in grapes and berries. Bone morphogenetic protein4 (BMP4) stimulates osteocalcin synthesis via p38 mitogenactivated protein (MAP) kinase in osteoblastlike MC3T3E1 cells. The present study aimed to investigate the effects of resveratrol on BMP4induced osteoprotegerin (OPG) synthesis in MC3T3E1 cells. Resveratrol alone had no effect on OPG expression levels, but significantly enhanced BMP4induced OPG release. In addition, resveratrol markedly amplified the mRNA expression levels of BMP4induced OPG. SB203580 is an inhibitor of p38 MAP kinase, which was shown to suppress BMP4stimulated OPG release. BMP4induced phosphorylation of p38 MAP kinase was also enhanced by resveratrol. Furthermore, SB203580 significantly reduced the resveratrolinduced amplification of BMP4stimulated OPG release. These results suggested that resveratrol was able to upregulate BMP4stimulated OPG synthesis via the amplification of p38 MAP kinase activity in osteoblasts.