Author: Zhao Shuzhen Wen Zhengfang Liu Shanshan Liu Ying Li Xiaorui Ge Yanna Li Shaoru
Publisher: Spandidos Publications
E-ISSN: 1791-3004|12|3|3923-3929
ISSN: 1791-2997
Source: Molecular Medicine Reports, Vol.12, Iss.3, 2015-09, pp. : 3923-3929
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Abstract
MicroRNAs (miRs) are a class of non-coding RNAs that function as key regulators of gene expression at the post-transcriptional level. miR-148a has been suggested to be associated with human ovarian cancer, however, the detailed functions of miR148a in ovarian cancer remain to be fully elucidated. The present study aimed to investigate the regulatory mechanism of miR-148a in ovarian cancer cells. Reverse transcriptionquantitative polymerase chain reaction and western blot analysis were conducted to examine the RNA and protein levels, respectively. The luciferase reporter assay was used to determine the target relationship. Cell proliferation and apoptosis assays were additionally conducted. The present study demonstrated that miR148a inhibited cell proliferation and promoted the paclitaxelinduced apoptosis of ovarian cancer cells. Furthermore, protein disulfide isomerase family A, member 3 (PDIA3) was identified as a target gene of miR148a. A fluorescent reporter assay was performed to confirm that miR148a was able to directly bind to the 3'untranslated region of PDIA3 mRNA. In addition, miR148a was frequently downregulated in ovarian cancer tissue, whereas the expression levels of PDIA3 were increased. Knockdown of PDIA3 significantly inhibited the proliferation and promoted the paclitaxelinduced apoptosis of the ovarian cancer cells, whereas overexpression of PDIA3 had the opposite effects. Therefore, the results of the present study suggested that miR148a inhibited the proliferation and promoted the paclitaxelinduced apoptosis of ovarian cancer cells, and this may be partly attributed to direct targeting of PDIA3.
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