Protective role of dietary‐supplemented selenium and vitamin E in heat‐induced apoptosis and oxidative stress in mice testes

Publisher： John Wiley & Sons Inc

E-ISSN： 1439-0272|47|10|1109-1119

ISSN： 0303-4569

Source： ANDROLOGIA (ELECTRONIC), Vol.47, Iss.10, 2015-12, pp. : 1109-1119

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Abstract

SummaryThis study evaluated the role of selenium (0.5 ppm selenium/kg diet) and vitamin E (200 mg alpha‐tocopherol/kg diet) on spermatogenesis after scrotal hyperthermia (42 °C, 30 min) in six different groups of male Balb/c mice; Control, Heat shock, Selenium, Selenium+heat shock, Vitamin E and Vitamin E+heat shock. Markers of the stress responses, hypoxia and oxidative stress, were evaluated in testis after the hyperthermic shock. Hyperthermia caused an elevated mRNA expression of hypoxia‐inducible factor‐1 alpha, haem oxygenase‐1 (HMOX‐1) and also glutathione peroxidase activity and reactive oxygen species (ROS). Apoptosis was evaluated by TUNEL assay and further by mRNA expression of Bcl‐2, caspase 3, 8, 9, bid and AKT. TUNEL assay showed significant increase in apoptotic index of spermatogenic cells, whereas decrease in mRNA expression of Bcl‐2, AKT and increase in caspase 3, 8, 9 and Bid in heat‐shock group were observed. A significant decrease in sperm motility was also seen in heat‐shock group in comparison with control group. These observations clearly indicate the development of oxidative stress and apoptosis after hyperthermia. Further analysis in Selenium+heat shock and Vitamin E+heat shock groups showed protective behaviour as compared to effects in heat‐shock group which could be of therapeutic interest in future studies.