Bioorthogonal SERS Nanoprobes for Mulitplex Spectroscopic Detection, Tumor Cell Targeting, and Tissue Imaging

E-ISSN： 1521-3765|21|37|12914-12918

ISSN： 0947-6539

Source： CHEMISTRY - A EUROPEAN JOURNAL, Vol.21, Iss.37, 2015-09, pp. : 12914-12918

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Abstract

AbstractA surface‐enhanced Raman scattering (SERS) technique shows extraordinary features for a range of biological and biomedical applications. Herein, a series of novel bioorthogonal SERS nanoprobes were constructed with Gold nanoflower (AuNF) and Raman reporters, the signals of which were located in a Raman‐silent region of biological samples. AS1411 aptamer was also co‐conjugated with AuNF through a self‐assembled monolayer coverage strategy. Multiplex SERS imaging using these nanoprobes with three different bioorthogonal small‐molecule Raman reporters is successfully achieved with high multiplexing capacity in a biologically Raman‐silent region. These Raman nanoprobes co‐conjugated with AS1411 showed high affinity for tumor cells with overexpressed nucleolin and can be used for selective tumor cell screening and tissue imaging.