Publisher： Trans Tech Publications
E-ISSN： 1662-7482|2015|737|374-380
ISSN： 1660-9336
Source： Applied Mechanics and Materials, Vol.2015, Iss.737, 2015-04, pp. : 374-380
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Abstract
We collected 17 soil samples from tobacco fields in Bijie City, Guizhou Province, Luzhou City, Sichuan Province. A total of 247 bacterial strains were isolated and 19 pairs of antagonism against Phytophthora nicotianae by tablet dual culture. Then six antagonistic and compatibility better bacterial strains were selected.. Further by antibacterial, pot and field experiments, come to the strain combination B41B57 and L7L47 have best inhibitory effect. At last, identified B41 as Bacillus subtilis, L7, L47 is B.amyloliquefaciens, B57 is Pseudomonas.putida by morphological observation, physiological and biochemical characteristics and 16SrDNA sequence analysis.
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