Publisher: Spandidos Publications
E-ISSN: 1792-1082|11|2|1207-1212
ISSN: 1792-1074
Source: Oncology Letters, Vol.11, Iss.2, 2016-01, pp. : 1207-1212
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Abstract
MicroRNAs (miRs) have been demonstrated to serve important roles in the development and progression of human cancer, primarily through the direct targeting of oncogenes or tumor suppressors. It has been previously suggested that miR126 may be associated with endometrial cancer (EC). However, the exact role of miR126 in the migration and invasion of EC cells has not yet been studied. The present study demonstrated that the expression of miR126 was significantly decreased in EC tissues when compared with matched normal adjacent tissues. The current study reverse transcription-quantitative polymerase chain reaction was performed in order to examine the expression level of miR-126. Wound healing and transwell assays were used to examine cell migration and invasion. A luciferase reporter assay was used to determine the targeting relationship and western blotting assay was performed to detect the protein expression. Furthermore, the overexpression of miR126 significantly inhibited EC SKOV3 cell migration and invasion. Molecular mechanism investigation established that insulin receptor substrate 1 (IRS1) functioned as a direct miR126 target, and its expression was negatively regulated by miR126 at a posttranscriptional level in the SKOV3 cells. Additionally, the overexpression of IRS1 reversed the inhibitory effect of miR126 overexpression on SKOV3 cell migration and invasion. In conclusion, the current study demonstrated that miR126 inhibited EC cell migration and invasion, at least partially through the direct targeting of IRS1, suggesting that miR126 may aid the treatment of EC metastasis.