Biochemical Cooperation Between Klebsiella oxytoca Sc and Methylobacterium mesophilium Sr for Complete Degradation of Dimethyl Isophthalate

Author： Li Jiaxi Gu Ji-Dong

Publisher： Springer Publishing Company

ISSN： 1567-7230

Source： Water, Air and Soil Pollution: Focus, Vol.6, Iss.5-6, 2006-12, pp. : 569-574

Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.

Previous Menu Next

Abstract

Klebsiella oxytoca Sc and Methylobacterium mesophilicum Sr were isolated from enrichment cultures using dimethyl isophthalate (DMI) as the sole carbon and energy source and mangrove sediment as an inoculum. Complete degradation of DMI required both species of bacteria at different biochemical transformation steps. The biochemical degradation pathway was DMI to monomethyl isophthalate (MMI) by K. oxytoca Sc, MMI to isophthalate (IPA) by M. mesophilium Sr, and IPA by both K. oxytoca Sc and M. mesophilium Sr sequentially. The consortium comprising of K. oxytoca Sc and M. mesophilicum Sr was effective in complete degradation of DMI in eight days. Our results suggest that a consortium of microorganisms indigenous to the mangrove environment is responsible for mineralization of environmental pollutant DMI through biochemical cooperation.